Analysis Note

Positive ControlLymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue

Application

ELISA (1:1000)r>Flow Cytometry (1:500)r>Immunofluorescence (1:100)r>Immunohistochemistry (1:100; see application references)r>Immunoprecipitation (1:100; see application references)r>Radioimmunoassay (1:5000)r>Immunoblotting (see application references)

General description

This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.

Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP₃ receptor subtypes.

Recognizes all of the ~260 kDa IP₃ receptor subtypes.

Immunogen

a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.

Specific for the C-terminal cytoplasmic domain of the IP₃ receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)